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Effect of OBNC microspheres on regulating MSCs. A) The expression of FAK and p-FAK detected by Western blot analysis. B) Quantitative analysis of the Western blot results. C) The expression of the integrin-α5 gene. D) The expression of the integrin-α8 gene. E) The expression of the Lamb-2 gene. F) The expression of the Spp-1 gene. G) The expression of the Vav-3 gene. H) The expression of the CDC-42 gene. I) Detection of the CDC-42 active protein by a <t>GLISA</t> kit. J) Detection of Rac-1 active protein by a GLISA kit. K) Detection of Rho A active protein by a GLISA kit. L) Mechanism of OBNC hydrogels triggering the membrane receptor switch and activating integrin receptors. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001).
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Effect of OBNC microspheres on regulating MSCs. A) The expression of FAK and p-FAK detected by Western blot analysis. B) Quantitative analysis of the Western blot results. C) The expression of the integrin-α5 gene. D) The expression of the integrin-α8 gene. E) The expression of the Lamb-2 gene. F) The expression of the Spp-1 gene. G) The expression of the Vav-3 gene. H) The expression of the CDC-42 gene. I) Detection of the CDC-42 active protein by a GLISA kit. J) Detection of Rac-1 active protein by a GLISA kit. K) Detection of Rho A active protein by a GLISA kit. L) Mechanism of OBNC hydrogels triggering the membrane receptor switch and activating integrin receptors. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001).

Journal: Bioactive Materials

Article Title: Mechanically sensitized hydrogel microspheres trigger membrane receptor switch for cartilage repair

doi: 10.1016/j.bioactmat.2026.03.017

Figure Lengend Snippet: Effect of OBNC microspheres on regulating MSCs. A) The expression of FAK and p-FAK detected by Western blot analysis. B) Quantitative analysis of the Western blot results. C) The expression of the integrin-α5 gene. D) The expression of the integrin-α8 gene. E) The expression of the Lamb-2 gene. F) The expression of the Spp-1 gene. G) The expression of the Vav-3 gene. H) The expression of the CDC-42 gene. I) Detection of the CDC-42 active protein by a GLISA kit. J) Detection of Rac-1 active protein by a GLISA kit. K) Detection of Rho A active protein by a GLISA kit. L) Mechanism of OBNC hydrogels triggering the membrane receptor switch and activating integrin receptors. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001).

Article Snippet: RhoA GLISA Activation Assay (No. BK124, Cytoskeleton, USA), CDC42 GLISA Activation Assay (No. BK127, Cytoskeleton, USA), and Rac1 GLISA Activation Assay (No. BK128, Cytoskeleton, USA) were used to measure the activation of the GTPase protein family.

Techniques: Expressing, Western Blot, Membrane